Antigen

Antigen Class

TFs and others

Antigen

Dnmt3a

Cell type

Cell type Class

Neural

Cell type

Cerebral Cortex

MeSH Description

Type of cerebral cortex which does not pass through a perinatal phase of six-layered structure as in the NEOCORTEX and develops into three or four layers in the mature brain. Allocortex has three subareas: archi- paleo- and periallo-cortex.

Sample

source_name

Cerebral cortex

tissue

Cerebral cortex

strain

C57BL/6

genotype

Dnmt3a1_delta_N

developmental stage

postnatal day 21

chip antibody

anti-DNMT3A

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The cerebral cortex was isolated, crushed and homogenized briefly by pipetting. Tissues were cross-linked in 1% formaldehyde and sheared with a Covaris M220 Ultrasonicator. ChIP-Seq Libraries were prepared with ThruPLEX® DNA-seq 48D Kit (Rubicon Genomics).

Sequencing Platform

instrument_model

NextSeq 2000

mm10

Number of total reads

53789194

Reads aligned (%)

82.1

Duplicates removed (%)

59.3

Number of peaks

204 (qval < 1E-05)

mm9

Number of total reads

53789194

Reads aligned (%)

82.0

Duplicates removed (%)

59.3

Number of peaks

180 (qval < 1E-05)